Clinical mastitis is a most common disease in dairy herds causing huge economic losses directly to farmers and indirectly to Indian dairy sector. However, systematic review and meta-analysis of prevalence of clinical mastitis in cows in India has not been published so far. The aim of the present study was to provide the pooled estimate of the prevalence of clinical mastitis in crossbred cows in India by conducting the literature search for the period of 1995-2014. Meta-analysis using data records of 17873 crossbred cows and 7737 udder quarters from total of 17 published studies was done in R software. It was found that the pooled estimates of clinical mastitis in crossbred cows under cow-basis and quarter-basis was 16.08% (95% CI 11.69, 21.72) and 11.71% (95% CI 6.60, 19.94), respectively. High variation in prevalence estimates between studies indicated that several factors infl uence occurrence of clinical mastitis. It is suggested that systematic review and meta-analysis using large number of studies and incorporating several factors can be effi cient tool to update the disease control strategy and will be best resource for researchers to improve future work.